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fluid phase plasma complement bb  (Quidel)


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    Structured Review

    Quidel fluid phase plasma complement bb
    Fluid Phase Plasma Complement Bb, supplied by Quidel, used in various techniques. Bioz Stars score: 96/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/pm37093125-119-0-18
    Average 96 stars, based on 184 article reviews
    fluid phase plasma complement bb - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Complement C3d/C4d Deposition on Platelets Correlates with 2'-O-Methoxyethyl Antisense Oligonucleotide-Induced Thrombocytopenia in Monkeys.
    Article Snippet: 2¢-O-Methoxyethyl antisense oligonucleotide (2¢-MOE ASO)-induced severe thrombocytopenia (TCP) [platelet (PLT) count <50 K/mL] was observed in the Asian-sourced cynomolgus monkeys with low incidence (2%–4% at doses >5 mg/kg/week).. The potential mechanisms for TCP were studied using the Mauritian-sourced cynomolgus monkeys, which were shown to be more susceptible to ASO-induced TCP, along with the Asiansourced animals.. ISIS 405879, a 2¢-MOE ASO, induced severe TCP (PLT <50 K/mL) in seven of nine Mauritian-sourced monkeys but not in the Asian-sourced monkeys after 16 weeks of treatment at 40 mg/kg/week.

    Article Title: The Local Complement Activation on Vascular Bed of Patients with Systemic Sclerosis: A Hypothesis-Generating Study
    Article Snippet: Serum and plasma samples were then aliquoted in 1.5 mL Eppendorf tubes and stored at -80°C until use. .. Concentrations of FH in serum and SC5b-9 in plasma were assessed using the Human Complement Factor H ELISA kit (Hycult Biotech, Uden, The Netherlands) and the MicroVue C5a Plus EIA (QUIDEL), respectively, according to the manufacturers’ instructions. ..

    Article Title: A randomized diet‐induced weight‐loss intervention reduces plasma complement C3 : Possible implication for endothelial dysfunction
    Article Snippet: .. Complement C3a was measured in EDTA plasma by ELISA (MicroVue C3a Plus EIA kit, Quidel Corp.) ( ). ..

    Article Title: A randomized diet-induced weight-loss intervention reduces plasma complement C3: Possible implication for endothelial dysfunction.
    Article Snippet: .. Complement C3a was measured in EDTA plasma by ELISA (MicroVue C3a Plus EIA kit, Quidel Corp.) (19). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Complement C3d/C4d Deposition on Platelets Correlates with 2'-O-Methoxyethyl Antisense Oligonucleotide-Induced Thrombocytopenia in Monkeys.
    Article Snippet: 2¢-O-Methoxyethyl antisense oligonucleotide (2¢-MOE ASO)-induced severe thrombocytopenia (TCP) [platelet (PLT) count <50 K/mL] was observed in the Asian-sourced cynomolgus monkeys with low incidence (2%–4% at doses >5 mg/kg/week).. The potential mechanisms for TCP were studied using the Mauritian-sourced cynomolgus monkeys, which were shown to be more susceptible to ASO-induced TCP, along with the Asiansourced animals.. ISIS 405879, a 2¢-MOE ASO, induced severe TCP (PLT <50 K/mL) in seven of nine Mauritian-sourced monkeys but not in the Asian-sourced monkeys after 16 weeks of treatment at 40 mg/kg/week.

    Article Title: Novel and recurrent mutations in the C1NH gene of Arab patients affected with hereditary angioedema.
    Article Snippet: Background: Autosomal dominant hereditary angioedema (HAE) results in episodes of subcutaneous edema in any body part and/or submucosal edema of the upper respiratory or gastrointestinal tracts.. This disorder is caused by mutations in the C1NH gene, many of which have been described primarily in European patients.. However, the genetic cause of HAE in Middle Eastern Arab patients has not yet been determined.

    Article Title: The Local Complement Activation on Vascular Bed of Patients with Systemic Sclerosis: A Hypothesis-Generating Study
    Article Snippet: Serum and plasma samples were then aliquoted in 1.5 mL Eppendorf tubes and stored at -80°C until use. .. Concentrations of FH in serum and SC5b-9 in plasma were assessed using the Human Complement Factor H ELISA kit (Hycult Biotech, Uden, The Netherlands) and the MicroVue C5a Plus EIA (QUIDEL), respectively, according to the manufacturers’ instructions. ..

    Article Title: A randomized diet‐induced weight‐loss intervention reduces plasma complement C3 : Possible implication for endothelial dysfunction
    Article Snippet: .. Complement C3a was measured in EDTA plasma by ELISA (MicroVue C3a Plus EIA kit, Quidel Corp.) ( ). ..

    Article Title: A randomized diet-induced weight-loss intervention reduces plasma complement C3: Possible implication for endothelial dysfunction.
    Article Snippet: .. Complement C3a was measured in EDTA plasma by ELISA (MicroVue C3a Plus EIA kit, Quidel Corp.) (19). ..

    other:

    Article Title: Early Detection of Malignant Pleural Mesothelioma in Asbestos-Exposed Individuals with a Noninvasive Proteomics-Based Surveillance Tool
    Article Snippet: Complement Factor 9 (MicroVue SC5b-9 EIA kit, Quidel Corporation, San Diego, CA USA), Factor IX (AssayMax Human Factor IX ELISA kit, AssayPro St. Charles, MO USA) and Human CXCL13 (Human CXCL13/BLC/BCA-1 Quantikine ELISA kit, R&D Systems, Minneapolis, MN USA) were analyzed in order to validate SOMAmer results in the 68 controls and 32 MMs in the blinded validation trial.



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    Quidel fluid phase plasma complement bb
    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and <t>complement-mediated</t> cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.
    Fluid Phase Plasma Complement Bb, supplied by Quidel, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/pm37093125-119-0-18
    Average 96 stars, based on 1 article reviews
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    Image Search Results


    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Journal: Clinical Cancer Research

    Article Title: KLRG1 Cell Depletion as a Novel Therapeutic Strategy in Patients with Mature T-Cell Lymphoma Subtypes

    doi: 10.1158/1078-0432.CCR-23-3504

    Figure Lengend Snippet: Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Article Snippet: Human male AB plasma as a source of human complement was obtained from Sigma-Aldrich.

    Techniques: Flow Cytometry Complement-Mediated Cytotoxicity Assay, Incubation, Positive Control, Flow Cytometry, Labeling, Derivative Assay, Reporter Assay, Expressing, CDC Assay